O tempora, O molars!: Investigating second molar stable isotope ratios of historical Dutch individuals from early medieval to early modern sites

In this study, five archaeological sites across the Netherlands from early medieval to early modern time periods were sampled to reconstruct past diet of the individuals found therein. The sites chosen for the study were Alkmaar, Arnhem, Eindhoven, Klaaskinderkerke and Zwolle. The focus of the diet reconstruction was to analyze the childhood and adolescent diet using stable isotope analysis of carbon (δ13C) and nitrogen isotopes (δ15N). Through the use of tissues which are underutilized in archaeological diet reconstruction, dental enamel (en) and dentine (co) in conjunction, derived from second molars, it was possible to obtain isotopic ratios representative of childhood diet (enamel) and adolescent diet (dentine collagen). The results obtained were then used to analyze trends between the sites, between assigned sex and statistical relationships which served to provide grounds for further improvement of the methodology. Sampling and analysis of enamel was 100% successful, while...Show moreIn this study, five archaeological sites across the Netherlands from early medieval to early modern time periods were sampled to reconstruct past diet of the individuals found therein. The sites chosen for the study were Alkmaar, Arnhem, Eindhoven, Klaaskinderkerke and Zwolle. The focus of the diet reconstruction was to analyze the childhood and adolescent diet using stable isotope analysis of carbon (δ13C) and nitrogen isotopes (δ15N). Through the use of tissues which are underutilized in archaeological diet reconstruction, dental enamel (en) and dentine (co) in conjunction, derived from second molars, it was possible to obtain isotopic ratios representative of childhood diet (enamel) and adolescent diet (dentine collagen). The results obtained were then used to analyze trends between the sites, between assigned sex and statistical relationships which served to provide grounds for further improvement of the methodology. Sampling and analysis of enamel was 100% successful, while sampling and analysis of collagen was 89% successful. The respective 76 and 68 samples were analyzed using a mass spectrometer and returned with reliable values. Results of intersite comparison aligned the, obtained results with past published literature regarding historical trends found in across the analyzed time periods, including increased consumption of marine fish in younger and more urban sites. Comparison of male and female assigned individuals has shown significant overlap between their values, suggesting that male and female individuals from the sites analyzed shared very similar diets during their childhood and adolescence. The investigation of isotope spacing of carbon values (Δen-co) allowed for determining that the diets of individuals analyzed was in large portion plant based, with significant inclusion of omnivore and marine foods as determined by isotopic ratios of carbon-13 and nitrogen-15. Analysis of statistical relationships between δ13Cen, δ13Cco and Δen-co has found a lack of correlation between δ13Cen δ13Cco, a positive correlation between Δen-co and δ13Cen and a negative correlation between Δen-co and δ13Cco. Follow-up studies should continue to investigate the recorded isotopic ratios in dental tissues, utilizing first and third molars as sources of further data capable of reconstructing sub-adult diet, as well as analyze local fauna to improve the accuracy of dietary reconstruction. The use of first and third molar isotopic ratios would also prove invaluable for investigating the statistical relationship between the tissues, due to the age of dental development and its relationship to diet reconstruction.Show less

Leiden University Student Repository

Documents

In Collections

O tempora, O molars!: Investigating second molar stable isotope ratios of historical Dutch individuals from early medieval to early modern sites