Magnetic tweezers experiments in low salt conditions and unzipping of double stranded DNA

Persistent URL of this record https://hdl.handle.net/1887/52796

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Physics (MSc)

Heinsman, Joost (2017)

Magnetic tweezers experiments in low salt conditions and unzipping of double stranded DNA

Master thesis | Physics (MSc)

The structure of chromatin fibers has been researched in different salt conditions with Magnetic tweezers. The drag force on the tether as a result of flowing in a new buffer into the flow cell resulted in structural changes of the chromatin and disassembly of the histone octamer. In a new flow cell design, the drag force was eliminated by changing the buffer conditions by diffusion through a permeable membrane made from PEG-DA 400. Furthermore, double stranded DNA was unzipped using Magnetic tweezers and the position of the melting force was correlated with the G-C content calculated from the sequence.