We introduce an open source, freely available algorithm for automatic correlation of fluorescence and electron microscopy images in Correlative Light-Electron Microscopy (CLEM). With a minimal...Show moreWe introduce an open source, freely available algorithm for automatic correlation of fluorescence and electron microscopy images in Correlative Light-Electron Microscopy (CLEM). With a minimal amount of manual input, two custom modules perform an automatic identification of the fluorescent nuclei and the watershed nucleus of a TEM image and hence calculate the necessary affine transformation for accurate correlation. The process can be performed on a group of pre-processed images and allows for an efficient solution around the data handling bottleneck encountered in the technique.Show less
