The commingling of human skeletal remains poses a recurrent challenge for osteoarchaeologists. Prior to any comprehensive analysis, the reassociation of these remains is required. Over the past...Show moreThe commingling of human skeletal remains poses a recurrent challenge for osteoarchaeologists. Prior to any comprehensive analysis, the reassociation of these remains is required. Over the past seven decades, numerous methodologies have emerged to address this challenge. However, each method exhibits limitations, prompting the necessity for the advancement of current techniques and the introduction of novel approaches. Among these evolving methods, the portable X-ray fluorescence technique stands out. Originally not designed for application on human remains, its appeal lies in its non-destructive nature and cost-effectiveness, drawing keen interest from osteoarchaeologists and forensic anthropologists. This study aims to evaluate the effectiveness of portable X-ray fluorescence in sorting commingled archaeological remains buried in the Netherlands. It involves the examination of 40 adults, 20 unearthed from Arnhem and 20 from Middenbeemster, dating back to the post-medieval period. The research initiates by investigating both intra-skeletal and inter-skeletal chemical variation, further exploring the potential factors contributing to these differences. Throughout the analysis, multiple sorting attempts are conducted to ensure a thorough exploration of the technique's capabilities. The results indicate statistically significant chemical variation within the same skeleton and among different individuals. In terms of intra-skeletal variation, from the 12 skeletal elements tested in each individual, only four pairs of bones (humerus–femur, rib–mandible, tibia–parietal, and tibia–rib) did not exhibit variation in the concentrations of chemical elements. With regard to inter-skeletal variation, 23 out of the 30 elements detected by the pXRF, and 14 out of the 15 ratios explored, demonstrated significant differences. Variation was also observed between males and females, as well as between sites. For the differences among the adult sub-groups that were noted, no generalizations can be made unless further research is conducted. Finally, the reassociation attempts reveal that the most successful sorting cases involved two individuals from different sites, utilizing principal components derived from ratios, while the tooth measurements were excluded. Key ratios influencing the sorting process included Mn/K, Mn/S, Mn/Fe, K/Fe, Sr/Ca, Ca/P, Pb/Ca, S/Sr, and Sr/Pb. While the method showed promise when a small number of individuals were involved, it lacked consistency. Therefore, it should be used in conjunction with other methods until further research is conducted.Show less