This project addresses two methods of bloodstain dating for forensic investigations. Firstly and briefly, AFM-based nanoindentation and probe-related uncertainties will be discussed. A self-written...Show moreThis project addresses two methods of bloodstain dating for forensic investigations. Firstly and briefly, AFM-based nanoindentation and probe-related uncertainties will be discussed. A self-written Python program to assist in the determination of the AFM probe's tip radius will be presented. The emphasis of this project lies on the second method: digital image analysis. Bloodstains in controlled environments (temperature and humidity) are imaged in time series. From the obtained images, the mean color values of the bloodstain in function of time are extracted. Two optical imaging time series are performed that provide a proof of concept: the color of bloodstains changes in function of time and this change is observable using a microscope camera and image processing with Python. The behavior of the color changes will be characterized by considering the changes in different color models (RGB, HSV, CMYK, CIELAB).Show less
